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Gut Microbiome Lab

Next-generation sequencing of bacterial 16S rDNA is performed at Gut Microbiome Laboratory.

Our microbiome sequencing pipeline includes:

  • DNA extraction from faecal, caecal and tissue samples
  • PCR amplification of bacterial 16S rDNA
  • Gel electrophoresis quality control of PCR products
  • Quantification of amplicon libraries with Qubit fluorometer
  • Paired-end Sequencing with 2x300 bp read lengths on a Miseq instrument, with an output of up to 15 Gb
  • Bioinformatical processing and statistical analysis of raw sequence data using QIIME and an Elastic Computer Cloud

The gut microbiota has an important role for host health and can affect energy absorption, metabolism and the immune system. The diet can modulate the gut microbiota and can be used as a tool to improve the gut microbiota composition.

We analyse and monitor gut microbiota changes after dietary interventions. In addition, we investigate involved mechanisms for host-microbiome interactions on health in different model systems and use microbiota mapping to tailor new food concepts for prevention of metabolic diseases.

We collaborate both nationally and internationally with research groups and industries who are interested in microbiome profiling in relation to dietary factors and health.

Coordinator: Frida Fåk

Diplococci
Diplococci. Photo: Frida Fåk.

Food for Health Science Centre
Lund University
Medicon Village
SE-223 81  Lund, Sweden

Tel +46(46)222 000 (switchboard)

VAT number: SE202100321101